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Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line International Journal of Morphology
Montiel-Eulefi,E; Jara,F; Toro,C; Garcés,M; Leal,P.
Poly (L-lactic acid) (PLA) nanoparticles have the approval of the main institutions for drugs administration and therapeutics. However, the use of lactic acid polymer is controversial because lactic acid has been proposed as an energy source for cancer cells. The aim of this study was to evaluate the cytotoxic, apoptotic and cell cycle properties of PLA and CuSO4-loaded PLA biodegradable nanoparticles on MKN-45 gastric adenocarcinoma cell line. PLA nanoparticles for the delivery of the anticancer active principle CuSO4 were obtained using the double emulsion method. PLA and CuSO4 loaded PLA nanoparticles were morphologically characterized and their size determined using transmission electron microscopy (TEM). The cytotoxicity of this drug delivery system...
Tipo: Journal article Palavras-chave: Copper loaded nanoparticles; Poly-lactic acid; Adenocarcinoma MKN45cells; Cytotoxicity; Cell cycle.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100011
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Epiblast Embryo Stem Cells Give Origin to Adult Pluripotent Cell Populations: Primordial Germ Cell, Pericytic and Haematopoyetic Stem Cells. A Review International Journal of Morphology
Montiel-Eulefi,E; Sánchez,R; Rojas,M; Bustos-Obregón,E.
Adult stem cells are great promise to the future of regenerative therapy, and understanding of its embryonic origin permit the discrimination of stem cell sources. Embryonic stem cells derived from inner cell mass of blastocyst originate the primordial germ cells, and pericyte stem cell associated to vessels endothelium in yolk sac. Currently, it is being proposed that embryonic primordial germ cell could originate hematopoietic stem cells based on the detection of germ cell markers (SSEA-1/TEC-1, Oct-4 and Nanog) in stem cell harvested from fetal liver and bone marrow. However, different experimental evidence points at two separate differentiation routes toward primordial germ cells, and hematopoietic stem cell with the same embryonic origin. The...
Tipo: Journal article Palavras-chave: Stem cell origin; Primordial gem cell; Perycitic stem cell; Haemangioblast.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400056
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